Diabetes and Obesity
Focus:
I am creating this module for use in the first semester of the Introductory Biology majors course. The idea is to implement CREATE in the lab portion. The lab portion is run with 24-30 students who meet once a week for 2.25 hrs during a 12 week semester. I wanted to create a module that was oriented to an issue that the students would be aware of but that would also teach through a wide variety of course associated topics. I chose diabetes and obesity. Papers are not sequential in topic or publication but rather follow a progression of topics that sequence with the lecture series as outline below. My intent is not to present the entire paper due to time constraints but rather to pick 1-2 key figures from each results and from each paper and have them go through all the CREATE tools using these abbreviated materials. Although it is not listed I would introduce the CREATE method using a the “baby face recognition” as a training article.
Overview:
Applicable for Courses:
Educational Level:
Introductory LevelRoadmap Objectives:
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- Article: Stanhope, Kimber L., et al. "Consuming fructose-sweetened, not glucose-sweetened, beverages increases visceral adiposity and lipids and decreases insulin sensitivity in overweight/obese humans." The journal of clinical investigation 119.5 (2009): 1322.
- Content area/major concepts: macromolecules, glycolysis
- Methods or technology used to obtain data:
- How the CREATE strategy was used:
- Biggest teaching challenge: It is pretty long and has a LOT of figures so it would definitely need to be abridged
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- Article: Pouli, Aristea E., et al. "Insulin targeting to the regulated secretory pathway after fusion with green fluorescent protein and firefly luciferase." Biochemical Journal 331.Pt 2 (1998): 669.
- Content area/major concepts: endomembrane system, cloning
- Methods or technology used to obtain data: cloning, fusion proteins, cell transfection, immunocytochemistry,
- How the CREATE strategy was used:
- Biggest teaching challenge:
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- Article: Roth, Richard A., and Delanie J. Cassell. "Insulin receptor: evidence that it is a protein kinase." Science 219.4582 (1983): 299-301. Or Kasuga, Masato, F. Anders Karlsson, and C. Ronald Kahn. "Insulin stimulates the phosphorylation of the 95,000-dalton subunit of its own receptor." Science215.4529 (1982): 185-187.
- Content area/major concepts: cell signal receptors, phosphorylation
- Methods or technology used to obtain data: phosphotyrosine antibody detection, gel electrophoresis, western blotting
- How the CREATE strategy was used:
- Biggest teaching challenge:
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- Article: Inoue, Mayumi, et al. "The exocyst complex is required for targeting of Glut4 to the plasma membrane by insulin." Nature 422.6932 (2003): 629-633.
- Content area/major concepts: membrane transport mechanisms (protein and vesicle mediated)
- Methods or technology used to obtain data: yeast two hybrid screen, expression construction, in vitro GST pull-down assay, immunoprecipitation and blotting, immunoflourescent microscopy
- How the CREATE strategy was used:
- Biggest teaching challenge:
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- Article: Everard, Amandine, et al. "Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity." Proceedings of the National Academy of Sciences 110.22 (2013): 9066-9071.
- Content area/major concepts: microbial diversity
- Methods or technology used to obtain data:
- How the CREATE strategy was used:
- Biggest teaching challenge: